Phylogenomics analysis pipeline by BuscoPhylo

Method

• • BUSCO predictions were performed on each genome using BUSCO V5 (Manni et al 2021) and OrthoDB V10 ( Zdobnov EM et al, 2020).

• • Alignments was performed using Muscle and trimAl (Edgar, 2004; Capella-Gutiérrez et al., 2009).

• • ML tree was inferred using IQ-TREE version 1.6.12 (Nguyen et al., 2015) with the model selection from ModelFinder (Kalyaanamoorthy et al., 2017) using the following defaults parameters: “-bb 1000 -alrt 1000 -nt AUTO -ntmax”.

• • The tree file is visualized using ETE Toolkit (Huerta-Cepas et al., 2016).

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